NEBuilder can be used to design primers for your Gibson Assembly reaction, based
on the entered fragment sequences and the polymerase being used for amplification.
To get started, please have the sequences of your DNA fragments ready in raw or FASTA format. NEBuilder has many common vector sequences available for you, as well as a few sample insert sequences if you just want to learn. You should also have ready either the restriction sites or sequence coordinates that will define the insertion site on the vector.Basic Usage: